0

I am using Blast command line. The query (length 9) is ADZTZZETK, but when blasted against database sequence, it gives alignment of length 8 (ADZTZZET:ADZTZZET). It means that last, 9th, character of query sequence, K, is mis-matched with database sequence. However I still want to get the 9th mis-matched character of database sequence. Could someone tell me which option I should use in command line blast for that?

4

1 回答 1

0

不幸的是，我认为您无法从 BLAST 获得完整的查询或主题序列。如果您查看命令行文档outfmt中的选项，最接近的选项是. 如果您可以下载格式的数据库，那么您可以单独解析该文件并获取主题序列。sseqAligned part of subject sequenceFASTA

于 2016-04-20T20:33:32.153 回答